منابع مشابه
Peptide synthesis: chemical or enzymatic
Abbreviations: CD: circular dichroism CLEC: cross linked enzyme crystals DDC: double dimer constructs ESI: electrospray ionization HOBT: hydroxybenzotriazole HPLC: high performance liquid hromatography KCS: kinetically controlled synthesis MALDI: matrix-assisted laser desorption ionization MAP: multiple antigen peptide system MS: mass spectrometry NMR: nuclear magnetic resonance SPS: solution p...
متن کاملEnzymatic Peptide Synthesis. Carboxypeptidase Y Catalyzed Formation of Peptide Bonds
It is demonstrated that carboxypeptidase Y from Saccharomyces cerevisiae can catalyze the formation of peptide bonds using N-acylamino acid esters as substrates and free amino acids or amino acid amides as nucleophiles, The coupling yields observed with free amino acids were max. 60 % for alanine and lysine and they depended strongly on the reaction parameters; viz. pH, temperature and concentr...
متن کاملEffect of Microwave Radiation on Enzymatic and Chemical Peptide Bond Synthesis on Solid Phase
Peptide bond synthesis was performed on PEGA beads under microwave radiations. Classical chemical coupling as well as thermolysin catalyzed synthesis was studied, and the effect of microwave radiations on reaction kinetics, beads' integrity, and enzyme activity was assessed. Results demonstrate that microwave radiations can be profitably exploited to improve reaction kinetics in solid phase pep...
متن کاملEnzymatic Synthesis of the Peptide in Bacterial Uridine Nucleotides
The pentapeptide moiety of uridine diphosphate acetylmuramyl-pentapeptide, a precursor of the peptidoglycan of bacterial cell walls, is synthesized in a reaction sequence involving five enzymes. The amount and specificity of these enzymes have been studied in 10 microorganisms, five of which contain L-lysine as the third amino acid in the peptide sequence, and five of which contain instead meso...
متن کاملSimple Preparation of Pacific Cod Trypsin for Enzymatic Peptide Synthesis
Trypsin from the pyloric caeca of Pacific cod (Gadus macrocephalus) was easily prepared by affinity chromatography on Benzamidine Sepharose 6B and gel filtration on Superdex 75. Pacific cod trypsin was composed of three isozymes, and their molecular masses were estimated 23,756.34 Da, 23,939.62 Da, and 24,114.81 Da by desorption/ionization time-of-flight mass spectroscopy (MALDI/TOF-MS) and the...
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ژورنال
عنوان ژورنال: Electronic Journal of Biotechnology
سال: 2007
ISSN: 0717-3458,0717-3458
DOI: 10.2225/vol10-issue2-fulltext-13